streptavidin sa sensor chips (Biacore)

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Biacore streptavidin sa sensor chips
Streptavidin Sa Sensor Chips, supplied by Biacore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/streptavidin+sensor+chip/chips+s+sa+series/us12397016-277-14-18
Average 86 stars, based on 1 article reviews

streptavidin sa sensor chips - by Bioz Stars, 2026-10

86/100 stars

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Article Title: Combinatorial synthesis of a hyaluronan based polysaccharide library for enhanced CD44 binding.
Article Snippet: HMW-HA (CAS Number: 9067-32-7, sodium salt from rooster comb, Sigma-Aldrich) was biotinylated using hydrazide biotin as previously described (Mahoney, Aplin, Calabro, Hascall, & Day, 2001), and immobilized onto a streptavidin SA Biacore Sensor Chip using the previously established method (Liu & Finzel, 2014).

Binding Assay:

Article Title: A cereblon (CRBN) molecular glue degrader of NIMA-related kinase 7 (NEK7) reveals a context-dependent role in NLRP3 inflammasome activation
Article Snippet: .. Binding of compounds to CRBN was assessed using SPR by immobilizing biotinylated DDB1:N-avi-CRBN complex (immobilized to ∼8000 RUs) on a Biacore streptavidin sensor chip and then injecting a dilution series of each compound. .. Experiments were performed with a Biacore T200 instrument with a 12 point, 2-fold dilution series and a variable top concentration, typically between 1 - 10 μM compound.

Article Title: A cereblon-based glue degrader of NEK7 regulates NLRP3 inflammasome in a context-dependent manner.
Article Snippet: Article

SPR Assay:

Article Title: A cereblon-based glue degrader of NEK7 regulates NLRP3 inflammasome in a context-dependent manner.
Article Snippet: Article

Article Title: Binding characterization of anthraquinone derivatives by stabilizing G-quadruplex DNA leads to an anticancerous activity
Article Snippet: .. Testing of SPR was carried out using the Biacore T200 instrument (optical biosensor system made by GE Healthcare, Chicago, IL) according to conventional protocols., , 10 nM 5′ biotin-labeled d-[AGGG(TTAGGG) 3 ] was diluted in HEPES buffer (100 mM KCl, 0.01 mM HEPES, 3 mM EDTA and 0.05% (v/v) surfactant P20), then immobilization process was done on a derivatized streptavidin sensor chip purchased from BIACORE SA, make GE Healthcare Life Sciences, UK). .. Using Biacore T200 assessment software, data were fitted and analyzed to determine the affinity constant ( K b ).

Concentration Assay:

Article Title: Discovery of Daraxonrasib (RMC-6236), a Potent and Orally Bioavailable RAS(ON) Multi-selective, Noncovalent Tri-complex Inhibitor for the Treatment of Patients with Multiple RAS-Addicted Cancers.
Article Snippet: .. AviTag-CypA was immobilized on a streptavidin sensor chip by diluting CypA stock solutions to a final concentration of 1uM or 0.2uM in assay buffer (10 mM HEPES-NaOH pH 7.4, 150 mM NaCl, 0.005% v/v Surfactant P20, 2% v/v DMSO) and then using the built in Biacore 8K protocol at a flow rate of 30uL/minute for 10 seconds.Varying compound concentrations, diluted in the assay buffer without added DMSO and injected from low to high, were S73 flowed over the chip in assay buffer for 20 to 30 seconds followed by a dissociation wash with assay buffer only for 180 seconds. ..

Article Title: Discovery of Daraxonrasib (RMC-6236), a Potent and Orally Bioavailable RAS(ON) Multi-selective, Noncovalent Tri-complex Inhibitor for the Treatment of Patients with Multiple RAS-Addicted Cancers.
Article Snippet: RAS binding affinity: The binding affinity of compound-bound CypA for the mutant oncogenic or wild-type RAS proteins (KD2) mentioned was assessed by SPR on a Biacore 8 K instrument. .. AviTagRAS [residues 1–169] was immobilized on a streptavidin sensor chip by diluting RAS stock solutions to a final concentration of 10nM in assay buffer (10 mM HEPES-NaOH pH 7.4, 150 mM NaCl, 0.005% v/v Surfactant P20, 0.5mM EDTA pH 8.0, 10uM GMPPNP, and 2% v/v DMSO) and then using the built in Biacore 8K protocol at a flow rate of 10uL/minute for 20 seconds targeting <= 40RU. ..

Injection:

Recombinant:

Article Title: V Region of IgG Controls the Molecular Properties of the Binding Site for Neonatal Fc Receptor.
Article Snippet: We used surface plasmon resonance–based technology (Biacore 2000 system; Biacore Cytiva, Uppsala, Sweden) to evaluate the kinetic constants characterizing the interactions of human IgG1 Abs with FcRn. .. The immobilization of human recombinant biotinylated FcRn was realized by high affinity capture on the surface of streptavidin sensor chip (Biacore Cytiva) that was precoated with streptavidin by the manufacturer. ..

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